样本形式

完整蛋白，多肽

样本要求

● 样本量：蛋白>1 mg

● 尽量避免使用表面活性剂（SDS、Triton-X）和无机盐

样本运输

独立密封干冰盒运输

实验流程选项

● 基本流程：nanoESI-MS/MS → 基于蛋白质骨架和N-连接糖部分选择性解离的数据库搜索与鉴定

● 选项：1）互补的N-连接糖分析；2）互补的含N-糖基化位点多肽分析；3）互补的完整N-糖肽分析

测试报告

● 实验步骤（中英文）

● 质谱仪采集的原始数据

● 完整N-糖蛋白数据库搜索引擎定性搜索结果

1）基于蛋白质骨架氨基酸链解离（N-连接糖部分保持完整，作为静态修饰处理）和完整N-糖蛋白数据库搜索的图形解离匹配。

2）基于N-连接糖部分解离（氨基酸链保持完整，作为静态修饰处理）和N-连接糖数据库搜索的图形解离匹配。

参考文献

1. Structure-specific N-glycoproteomics characterization of NIST monoclonal antibody reference material 8671. Journal of Proteome Research, 2022, DOI: 10.1021/acs.jproteome.2c00027.

2. Exploration of quantitative site-specific serum O-glycoproteomics with isobaric labelling for the discovery of putative O-glycoprotein biomarkers. Proteomics-Clinical Applications, 2022, prca.202100095.

3. Recent advances in mass spectrometry-based approaches for proteomics and biologics: Great contribution for developing therapeutic antibodies. Pharmacology & Therapeutics, 2018, 185, 147-154.

4. Characterization of glycosylation in monoclonal antibodies and its importance in therapeutic antibody development. Critical Reviews in Biotechnology, 2021, 41, 300-315.

5. Bioanalytical methods for therapeutic monoclonal antibodies and antibody-drug conjugates: A review of recent advances and future perspectives. Journal Of Pharmaceutical And Biomedical Analysis, 2020, 179, 112991.

生物药糖基化修饰分析