生物药N/C端测序

样本形式

完整蛋白，多肽（状态：干粉、溶液）

样本要求

● 样本量：蛋白>1 mg，纯度>90%

● 尽量避免使用表面活性剂（SDS、Triton-X）和无机盐；挥发无机盐<20 mM，不挥发无机盐<5 mM

样本运输

独立密封干冰盒运输

实验流程选项

● 基本流程：蛋白酶酶切 → RPLC-nanoESI-MS/MS → 数据库搜索与鉴定

● 选项：N端使用Edman化学降解法测序

测试报告

● 实验步骤（中英文）

● 质谱仪采集的原始数据

● 生物药N/C端序列信息

参考文献

1. Structure-specific N-glycoproteomics characterization of NIST monoclonal antibody reference material 8671. Journal of Proteome Research, 2022, DOI: 10.1021/acs.jproteome.2c00027.

2. Exploration of quantitative site-specific serum O-glycoproteomics with isobaric labelling for the discovery of putative O-glycoprotein biomarkers. Proteomics-Clinical Applications, 2022, prca.202100095.

3. Recent advances in mass spectrometry-based approaches for proteomics and biologics: Great contribution for developing therapeutic antibodies. Pharmacology & Therapeutics, 2018, 185, 147-154.

4. Characterization of glycosylation in monoclonal antibodies and its importance in therapeutic antibody development. Critical Reviews in Biotechnology, 2021, 41, 300-315.

5. Bioanalytical methods for therapeutic monoclonal antibodies and antibody-drug conjugates: A review of recent advances and future perspectives. Journal Of Pharmaceutical And Biomedical Analysis, 2020, 179, 112991.