PRM靶向蛋白质组学

PRM（平行反应监测，parallel reaction monitoring）是一种基于LC-M  S/MS的蛋白质靶向分析技术。相较于需要预先准备无干扰的碎片离子的传统选择反应监测 (SRM)，PRM监测二级质谱中的所有碎片离子，正得到越来越广泛的应用。通过目标肽段或修饰肽段的预先设定的靶向选择性检测，可获得高灵敏和高准确的相对或绝对定量分析。

实验流程选项

● 基本流程：发现组学 → 目标肽段列表 → 样本多肽制备 → PRM方法建立与评价 → 目标肽段PRM分析 → 目标肽段相对/绝对定量

测试报告

● 实验步骤（中英文）

● 质谱仪采集的原始数据

● 目标肽段相对/绝对定量结果

1）列表输出：目标肽段原始强度、相对/绝对定量结果

2）可视化视图呈现：目标肽段二级质谱图

参考文献

1. A mass spectrometry-based targeted assay for detection of SARS-CoV-2 antigen from clinical specimens. EBio Medicine, 2021, 69,103465.

2. Proteome analysis of urinary biomarkers in a bovine IRBP-induced uveitis rat model via data-independent acquisition and parallel reaction monitoring proteomics. Frontiers in Molecular Biosciences, 2022, 9, 831632.

3. Development of a parallel reaction monitoring mass spectrometry assay for the detection of SARS-CoV-2 spike glycoprotein and nucleoprotein. Analytical Chemistry, 2020, 92, 13813-13821.

4. Fully integrated protein absolute quantification platform for analysis of multiple tumor markers in human plasma. Talanta, 2021,226, 122102.

5. Alternative LC-MS/MS platforms and data acquisition strategies for proteomic genotyping of human hair shafts. Journal of Proteome Research, 2021,20,4655-4666.